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human transcription factors joung  (Addgene inc)


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    Structured Review

    Addgene inc human transcription factors joung
    Human Transcription Factors Joung, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+transcription+factors/pJBL6662+(Plasmid+%23139139)/pm41435820-1666-111-117
    Average 93 stars, based on 2 article reviews
    human transcription factors joung - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Derivation of Canine Induced Pluripotent Stem Cells.
    Article Snippet: Animal Health Trust, Kentford, Newmarket, Suffolk, UK

    Article Title: Equine Induced Pluripotent Stem Cells have a Reduced Tendon Differentiation Capacity Compared to Embryonic Stem Cells
    Article Snippet: Fibroblast cells were passaged at confluency with trypsin-EDTA (Sigma) for expansion, and stocks were frozen in media with 10% DMSO. iPSC generation was performed as described in Ref. ( ). .. Briefly, 4 × 10 5 packaging cells (phoenix gag-pol cells) were plated onto five 6 cm plates (in DMEM, 10% fetal calf serum, 2 mM l -glutamine) and incubated overnight at 37°C, 5% CO 2 prior to transfection with plasmid vectors containing 3 μg of one of the following human transcription factors: pMXs.hOct 4 (Addgene 17217), pMXs.hSox2 (Addgene 17218), pMXs.hKlf4 (Addgene 17219), and pMXs.hc-myc (Addgene 17220), or with 3 μg of pMX.GFP (Cell Biolabs) to monitor efficiency of the initial transfection of the packaging cells and the subsequent viral transduction of the equine cells. .. The viral vectors were pseudotyped with the VSVg envelope protein by including 3 μg of pVPack-VSV-G (Stratagene, Cheshire, UK) to each transfection reaction.

    Transfection:

    Article Title: Derivation of Canine Induced Pluripotent Stem Cells.
    Article Snippet: Animal Health Trust, Kentford, Newmarket, Suffolk, UK

    Article Title: Equine Induced Pluripotent Stem Cells have a Reduced Tendon Differentiation Capacity Compared to Embryonic Stem Cells
    Article Snippet: Fibroblast cells were passaged at confluency with trypsin-EDTA (Sigma) for expansion, and stocks were frozen in media with 10% DMSO. iPSC generation was performed as described in Ref. ( ). .. Briefly, 4 × 10 5 packaging cells (phoenix gag-pol cells) were plated onto five 6 cm plates (in DMEM, 10% fetal calf serum, 2 mM l -glutamine) and incubated overnight at 37°C, 5% CO 2 prior to transfection with plasmid vectors containing 3 μg of one of the following human transcription factors: pMXs.hOct 4 (Addgene 17217), pMXs.hSox2 (Addgene 17218), pMXs.hKlf4 (Addgene 17219), and pMXs.hc-myc (Addgene 17220), or with 3 μg of pMX.GFP (Cell Biolabs) to monitor efficiency of the initial transfection of the packaging cells and the subsequent viral transduction of the equine cells. .. The viral vectors were pseudotyped with the VSVg envelope protein by including 3 μg of pVPack-VSV-G (Stratagene, Cheshire, UK) to each transfection reaction.

    Plasmid Preparation:

    Article Title: Derivation of Canine Induced Pluripotent Stem Cells.
    Article Snippet: Animal Health Trust, Kentford, Newmarket, Suffolk, UK

    Article Title: Equine Induced Pluripotent Stem Cells have a Reduced Tendon Differentiation Capacity Compared to Embryonic Stem Cells
    Article Snippet: Fibroblast cells were passaged at confluency with trypsin-EDTA (Sigma) for expansion, and stocks were frozen in media with 10% DMSO. iPSC generation was performed as described in Ref. ( ). .. Briefly, 4 × 10 5 packaging cells (phoenix gag-pol cells) were plated onto five 6 cm plates (in DMEM, 10% fetal calf serum, 2 mM l -glutamine) and incubated overnight at 37°C, 5% CO 2 prior to transfection with plasmid vectors containing 3 μg of one of the following human transcription factors: pMXs.hOct 4 (Addgene 17217), pMXs.hSox2 (Addgene 17218), pMXs.hKlf4 (Addgene 17219), and pMXs.hc-myc (Addgene 17220), or with 3 μg of pMX.GFP (Cell Biolabs) to monitor efficiency of the initial transfection of the packaging cells and the subsequent viral transduction of the equine cells. .. The viral vectors were pseudotyped with the VSVg envelope protein by including 3 μg of pVPack-VSV-G (Stratagene, Cheshire, UK) to each transfection reaction.

    Transduction:

    Article Title: Derivation of Canine Induced Pluripotent Stem Cells.
    Article Snippet: Animal Health Trust, Kentford, Newmarket, Suffolk, UK

    Article Title: Equine Induced Pluripotent Stem Cells have a Reduced Tendon Differentiation Capacity Compared to Embryonic Stem Cells
    Article Snippet: Fibroblast cells were passaged at confluency with trypsin-EDTA (Sigma) for expansion, and stocks were frozen in media with 10% DMSO. iPSC generation was performed as described in Ref. ( ). .. Briefly, 4 × 10 5 packaging cells (phoenix gag-pol cells) were plated onto five 6 cm plates (in DMEM, 10% fetal calf serum, 2 mM l -glutamine) and incubated overnight at 37°C, 5% CO 2 prior to transfection with plasmid vectors containing 3 μg of one of the following human transcription factors: pMXs.hOct 4 (Addgene 17217), pMXs.hSox2 (Addgene 17218), pMXs.hKlf4 (Addgene 17219), and pMXs.hc-myc (Addgene 17220), or with 3 μg of pMX.GFP (Cell Biolabs) to monitor efficiency of the initial transfection of the packaging cells and the subsequent viral transduction of the equine cells. .. The viral vectors were pseudotyped with the VSVg envelope protein by including 3 μg of pVPack-VSV-G (Stratagene, Cheshire, UK) to each transfection reaction.

    Retroviral:

    Article Title: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model.
    Article Snippet: .. Generation of feline iPSCs using retroviral (RV) vectors Retroviral plasmids based on the Moloney murine leukemia virus (MMLV) were purchased from Addgene and contained the coding sequences for the human transcription factors: OCT4 (Addgene #17217), SOX2 (Addgene #17218), NANOG (Addgene #18115), c-MYC (Addgene #17220), and KLF4 (Addgene #17219). .. For production of MMLV virus, platinum-A packaging cells (Catalog Number: RV-102, Cell Biolabs, San Diego, California) were plated 24 h before transfection in T25 cell culture flasks dishes at a density of 1 × 105 cells/cm2, one dish per retroviral vector plasmid.

    Article Title: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model
    Article Snippet: .. Retroviral plasmids based on the Moloney murine leukemia virus (MMLV) were purchased from Addgene and contained the coding sequences for the human transcription factors: OCT4 (Addgene #17217), SOX2 (Addgene #17218), NANOG (Addgene #18115), c-MYC (Addgene #17220), and KLF4 (Addgene #17219). .. For production of MMLV virus, platinum-A packaging cells (Catalog Number: RV-102, Cell Biolabs, San Diego, California) were plated 24 h before transfection in T25 cell culture flasks dishes at a density of 1 × 10 5 cells/cm 2 , one dish per retroviral vector plasmid.

    Virus:

    Article Title: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model.
    Article Snippet: .. Generation of feline iPSCs using retroviral (RV) vectors Retroviral plasmids based on the Moloney murine leukemia virus (MMLV) were purchased from Addgene and contained the coding sequences for the human transcription factors: OCT4 (Addgene #17217), SOX2 (Addgene #17218), NANOG (Addgene #18115), c-MYC (Addgene #17220), and KLF4 (Addgene #17219). .. For production of MMLV virus, platinum-A packaging cells (Catalog Number: RV-102, Cell Biolabs, San Diego, California) were plated 24 h before transfection in T25 cell culture flasks dishes at a density of 1 × 105 cells/cm2, one dish per retroviral vector plasmid.

    Article Title: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model
    Article Snippet: .. Retroviral plasmids based on the Moloney murine leukemia virus (MMLV) were purchased from Addgene and contained the coding sequences for the human transcription factors: OCT4 (Addgene #17217), SOX2 (Addgene #17218), NANOG (Addgene #18115), c-MYC (Addgene #17220), and KLF4 (Addgene #17219). .. For production of MMLV virus, platinum-A packaging cells (Catalog Number: RV-102, Cell Biolabs, San Diego, California) were plated 24 h before transfection in T25 cell culture flasks dishes at a density of 1 × 10 5 cells/cm 2 , one dish per retroviral vector plasmid.



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    Image Search Results


    ( A ) RT-qPCR analysis of Sp4 mRNA [normalized to glyceraldehyde-3-phosphate dehydrogenase ( GAPDH )] in buccal cell extracts from patients with SCZ ( n = 23) or CTRL subjects ( n = 22). *** P = 0.0009 as determined by unpaired t test with Welch’s correction for unequal variances. ( B ) RT-qPCR analysis of NOS1AP mRNA (normalized to GAPDH ) in buccal cell extracts from patients with SCZ ( n = 20) or CTRL subjects ( n = 23). No changes were observed ( P = 0.59) as determined by unpaired t test. ns, not significant. ( C ) RT-qPCR analysis of Dexras1 mRNA (normalized to GAPDH ) in buccal cell extracts from patients with SCZ ( n = 20) or CTRL subjects ( n = 22). No changes were observed ( P = 0.43) as determined by an unpaired t test with Welch’s correction for unequal variances. Error bars represent SD. For all analyses, outliers were removed [(A) = 4, (B) = 6, and (C) = 9] using the ROUT method ( Q = 1%), and samples with threshold cycle (Ct) values below detection levels or without an age-, race-, and gender-matched CTRL were removed.

    Journal: Science Advances

    Article Title: Differential SP4 expression and HSP60 abundance in buccal swabs from patients with schizophrenia

    doi: 10.1126/sciadv.aeb0460

    Figure Lengend Snippet: ( A ) RT-qPCR analysis of Sp4 mRNA [normalized to glyceraldehyde-3-phosphate dehydrogenase ( GAPDH )] in buccal cell extracts from patients with SCZ ( n = 23) or CTRL subjects ( n = 22). *** P = 0.0009 as determined by unpaired t test with Welch’s correction for unequal variances. ( B ) RT-qPCR analysis of NOS1AP mRNA (normalized to GAPDH ) in buccal cell extracts from patients with SCZ ( n = 20) or CTRL subjects ( n = 23). No changes were observed ( P = 0.59) as determined by unpaired t test. ns, not significant. ( C ) RT-qPCR analysis of Dexras1 mRNA (normalized to GAPDH ) in buccal cell extracts from patients with SCZ ( n = 20) or CTRL subjects ( n = 22). No changes were observed ( P = 0.43) as determined by an unpaired t test with Welch’s correction for unequal variances. Error bars represent SD. For all analyses, outliers were removed [(A) = 4, (B) = 6, and (C) = 9] using the ROUT method ( Q = 1%), and samples with threshold cycle (Ct) values below detection levels or without an age-, race-, and gender-matched CTRL were removed.

    Article Snippet: All experiments were performed once except the RT-qPCR for Sp4 mRNA for Origene primers due to limited buccal cell material.

    Techniques: Quantitative RT-PCR

    ( A ) Schematic of the four trial types in the AX-CPT task, with AX being the target. ( B ) Mean reaction time by trial type. Multiple unpaired t tests with Welch’s correction were used to determine differences by trial type. Adjusted P values were calculated using the Holm-Šídák method. Significant differences in reaction times were seen across all trial types ( P < 0.001). ( C ) Mean error rate by trial type. Multiple unpaired t tests with Welch’s correction were used to determine differences by trial type. Adjusted P values were calculated using the Holm-Šídák method. No significant differences between patients with SCZ and CTRL subjects were observed for the AY ( P = 0.33) and BY ( P = 0.79) trials. However, patients with SCZ had significantly higher mean error rates in the AX ( P = 0.02) and BX ( P = 0.01) trials. ( D ) Schematic of trial progression in the HVLT-R. Adapted image from “Timeline (Layout 5 × 1)” in BioRender templates (freely available) and drawn in Powerpoint. Unpaired t tests were used to determine P values; Welch’s correction was used in cases of unequal variance. Outliers (recognition n = 1) were removed using the ROUT method ( Q = 1%). Patients with SCZ scored lower in the categories of total recall ( P = 0.004), delayed recall ( P = 0.0005), retention ( P = 0.012), and recognition ( P < 0.0001). Error bars represent SD. ( E ) Increased Sp4 expression significantly correlates with lower recognition determination scores in the HVLT-R task as determined by Pearson’s r , two-tailed [ r (45) = −0.431, P = 0.012]. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Science Advances

    Article Title: Differential SP4 expression and HSP60 abundance in buccal swabs from patients with schizophrenia

    doi: 10.1126/sciadv.aeb0460

    Figure Lengend Snippet: ( A ) Schematic of the four trial types in the AX-CPT task, with AX being the target. ( B ) Mean reaction time by trial type. Multiple unpaired t tests with Welch’s correction were used to determine differences by trial type. Adjusted P values were calculated using the Holm-Šídák method. Significant differences in reaction times were seen across all trial types ( P < 0.001). ( C ) Mean error rate by trial type. Multiple unpaired t tests with Welch’s correction were used to determine differences by trial type. Adjusted P values were calculated using the Holm-Šídák method. No significant differences between patients with SCZ and CTRL subjects were observed for the AY ( P = 0.33) and BY ( P = 0.79) trials. However, patients with SCZ had significantly higher mean error rates in the AX ( P = 0.02) and BX ( P = 0.01) trials. ( D ) Schematic of trial progression in the HVLT-R. Adapted image from “Timeline (Layout 5 × 1)” in BioRender templates (freely available) and drawn in Powerpoint. Unpaired t tests were used to determine P values; Welch’s correction was used in cases of unequal variance. Outliers (recognition n = 1) were removed using the ROUT method ( Q = 1%). Patients with SCZ scored lower in the categories of total recall ( P = 0.004), delayed recall ( P = 0.0005), retention ( P = 0.012), and recognition ( P < 0.0001). Error bars represent SD. ( E ) Increased Sp4 expression significantly correlates with lower recognition determination scores in the HVLT-R task as determined by Pearson’s r , two-tailed [ r (45) = −0.431, P = 0.012]. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: All experiments were performed once except the RT-qPCR for Sp4 mRNA for Origene primers due to limited buccal cell material.

    Techniques: Expressing, Two Tailed Test

    ( A to C ) Increased Sp4 expression significantly correlates with an increased presence of positive (A), negative (B) symptoms, and excitement (C) as determined by Pearson’s r , two-tailed [ r (45) = 0.393, P = 0.026; r (45) = 0.329, P = 0.046; r (45) = 0.379, P = 0.026, respectively].

    Journal: Science Advances

    Article Title: Differential SP4 expression and HSP60 abundance in buccal swabs from patients with schizophrenia

    doi: 10.1126/sciadv.aeb0460

    Figure Lengend Snippet: ( A to C ) Increased Sp4 expression significantly correlates with an increased presence of positive (A), negative (B) symptoms, and excitement (C) as determined by Pearson’s r , two-tailed [ r (45) = 0.393, P = 0.026; r (45) = 0.329, P = 0.046; r (45) = 0.379, P = 0.026, respectively].

    Article Snippet: All experiments were performed once except the RT-qPCR for Sp4 mRNA for Origene primers due to limited buccal cell material.

    Techniques: Expressing, Two Tailed Test